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buffers, stains, markers

Fluorescent Protein Stains

Cleaver Scientific is pleased to launch 3 new fluorescent stains for protein detection that are both

reversible and more sensitive than many of the protein stains currently on the market. Available in blue,

green and red colours as CSLblue, CSLgreen and CSLred, these protein stains can detect a minimum

1-3ng of protein per square millimetre of gel. Staining and destaining times are comparable to those

required for standard Coomassie and SYPRO Ruby, while each gel may be visualised using a UV

transilluminator and visible light filter. Each stain’s metal chelate composition guarantees stability

without loss of sensitivity for at least 1 year if stored within the refrigerator.

Colorimetric Protein Stains

DyeHard is a new Coomassie Blue polymer stain whose sensitivity is comparable to silver stain. Its high

protein-binding affinity and specificity allows detection of as little as 3ng protein with minimal

background. Staining with DyeHard is faster and more convenient than standard Coomassie, since no

destain is required. DyeHard is also cost-effective, odourless and easy to dispose within the laboratory.

Protein Markers

Stable for 2 years if kept at -20°C, Cleaver Scientific PINK Plus prestained protein ladder includes three

conspicuous blue reference bands, 10, 40 and 90 kDa in size, for easy identification. Ranging from 10

to 175 kDa, these markers are premixed with loading dye for direct loading on gels. Cleaver Scientific

BLUE Wide Range Protein Ladders are also available for the separation of proteins greater than 175kDa

in size. Sharp green and red reference bands, 25 and 75kDa in size, enable easy identification of protein

bands within the gel up to a maximum size of 245kDa. These markers are supplied in gel loading buffer,

ready for immediate use.

PAGE buffers

Five buffers are available in powder sachets for a range of native and denaturing protein gel

electrophoresis techniques. Each powder sachet, which is supplied as a 10-pack, may be reconstituted

to make 1 litre of working solution. Running buffers are also supplied in 1 litre and 5 litre volumes as

ready made 10x Tris-Glycine and 10x Tris-Glycine-SDS solutions.

Ordering Information

Fluorescent Protein Stain

CSL-GREEN Green Protein Stain, 1 litre

CSL-RED Red Protein Stain, 1 litre

CSL-BLUE Blue Protein Stain, 1 litre

Protein Staining Solution

300-692-500 DyeHard Protein Staining solution - 500ml

300-692-1000 DyeHard Protein Staining solution - 1000ml

Protein Markers

CSL-PPL CSL Pink Plus Prestained Protein Ladder (10-175 kDa)

CSL-BBL Blue Wide Range Protein Ladder, (10-245 kDa)

Powdered Buffers

CSL-TGSDSP Powdered Tris-Glycine-SDS Running buffer - 10 Pouches (10 litres/pk)

CSL-TGP Powdered Tris-Glycine Running buffer - 10 Pouches (10 litres/pk)

CSL-TTSDSP Powdered Tris-TRICINE-SDS Running buffer - 10 Pouches (10 litres/pk)

CSL-MSDSP Powdered MOPS-SDS buffer Running buffer - 10 Pouches (10 litres/pk)

CSL-MESDSP Powdered MES-SDS buffer Running buffer - 10 Pouches (10 litres/pk)

Buffers for Protein Electrophoresis

TG10X1L Cleaver Buffer Tris-Glycine 10x 1 litre

TG10X5L Cleaver Buffer Tris-Glycine 10x 5 litre

TG-SDS10X1L Cleaver Buffer Tris-Glycine SDS 10x 1 litre

TG-SDS10X5L Cleaver Buffer Tris-Glycine SDS 10x 5 litre

Code CSLblue CSLgreen CSLred

Description Blue colorimetric protein stain, 1L Green fluorescent protein stain, 1L Red fluorescent protein stain, 1L

Documentation 312nm UV with white light filter 365nm UV with 530nm filter 312nm UV with 590nm filter

Sensitivity 3-5ng/mm

1-3ng/mm

Application Protein gels Protein gels and blots Protein gels

Technical Specifications

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