MaxPower Corporation CoolCube 10 Spezifikationen PDF herunterladen (Seite 18)

CSL-COM10 Comet Assay Tank for 10 Slides

CSL-COM20 Comet Assay Tank for 20 Slides

CSL-COM40 Comet Assay Tank for 40 Slides

CSL-COM80 Comet Assay Tank for 80 Slides

CSL-CHILLER Chiller unit for active slide temperature control

Comet Assay IV DNA damage and repair software scoring system

(contact sales@cleaverscientific.com)

F250 Julabo Chiller

Ordering Information

■ For Single Cell Gel Electrophoresis

■ Minimise exposure to light and reduce

background DNA damage

■ High efficiency cooling for enhanced

resolution

comet assay tanks

The Comet Assay

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells

exposed to γ-irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA

lesions and repair processes.

Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of

supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are

separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a

‘comet’ as its negatively charged broken ends and fragments migrate towards the anode during

electrophoresis.

Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and

embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis

in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is

electrophoresed, stained and then analysed using software such as Perceptive Instruments Comet

Assay IV.

Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged

cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The

head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is

concentrated within and towards the tail.

Cleaver Scientific recommends Comet Assay IV scoring software from Perceptive Instruments. Please

enquire by emailing [email protected].

Cleaver Scientific COMET assay tanks are available in four slide formats to study single cell gel

electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the

detection and quantitation of DNA damage in cells. Each tank’s robust construction from ebony acrylic

ensures that cells remain free of exposure to background light and DNA damage during electrophoresis,

while a cooled central platform provides a convenient surface for slide preparation and control of slide

temperature during the assay. Following electrophoresis DNA damage may be measured using the

highly recommended Comet Assay IV Software from Perceptive Instruments.

F250 Chiller

With PID control ensuring temperature stability within ±0.5°C, the F250 is the perfect recirculating

chiller for active slide temperature control. This chiller also benefits from a maximum flow rate of 15

litres per minute and 0.35 bar pressure, while a small footprint and frontal vents allow multiple units to

be aligned side-by-side if required. A versatile working temperature range of +5°C to +40°C makes the

chiller suitable for use in other applications such as flat-bed isoelectric focusing.

Full Screen Shot of a Comet Profile within the Comet Assay

IV Software.

A. The Comet ‘Head’ comprising largely intact genomic DNA.

B. The Comet ‘Tail’ of mobile damaged or fragmented DNA.

1 2 ... 13 14 15 16 17 18 19 20 21 22 23 ... 51 52

Kommentare zu diesen Handbüchern

Keine Kommentare

MaxPower Corporation CoolCube 10 Spezifikationen Seite 18

Kommentare zu diesen Handbüchern

Verwandte Produkte und Handbücher für Split-System-Klimaanlagen MaxPower Corporation CoolCube 10