MaxPower Corporation CoolCube 10 Spezifikationen Seite 36

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36
blotting
floating lid
filter paper
membrane
gel stack
absorbent
towelling
buffer
reservoir
porous
support
permanent
wick
Principle:
Through capillary action, the buffer travels
from the reservoir to the absorbent
towelling via the porous supports,
permanent wick, gel and membrane. This
action optimises the transfer of low and
high Molecular Weight DNA/RNA from gel
to membrane without mechanical damage
to even the thinnest of gels.
These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units
incorporate optimised permanent wicks which eliminate the requirement for overlapping wicks, offering
greater consistency.
The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for
faster transfer times.
Side by side transfer of multiple gels can be performed in a single set up.
Available in 15 x 26cm and 28 x 26cm formats.
CAPILLARY BLOTTERS
DOT AND SLOT BLOTTERS
Permanent wick with optimised
capillary action
Efficient transfer of high and low
M.Wt. fragments
Fast, easy set-up
DNA transfer in 2 hours or less;
RNA transfer in 3 hours or less
Floating lid ensures even
pressure without gel
compression
U
NIT DIMENSIONS
M
INI
30
X
30
X
10
CM
W
X
D
X
H) M
AXI
42
X
30
X
10
CM
Technical Specifications
Four different sample number size and style of
Hybridisation Manifold are offered:- two types of
dot blotter and two types of slot blotter.
Typical applications include clone screening with
DNA / RNA probes in Southern/Northern blots and
immunological screening with antibodies in
western blots.
The units incorporate precision lapped surfaces
to ensure uniform blotting membrane contact and
a leak proof gasket. These prevent lateral transfer
of samples – smudging – by ensuring that a
complete vacuum is formed. Six thumbscrews
ensure even and tight sealing for fast sample
suck down.
Dot blotters are available in 48 and 96 well
versions and slot blotters in 24 and 48 well.
Low cost
Simple construction
Easy assembly
Four sample configurations
Alpha-numeric sample
identification
M
ODEL
CSL-D48 CSL-D96 CSL-S24 CSL-S48
C
ONFIGURATION
3
X
16 8
X
12 2
X
12 3
X
16
S
IZE OF WELL
6
MM DIAMETER
6
MM DIAMETER
6
X
0.5
MM
6
X
0.5
MM
12
MM DEEP
12
MM DEEP
12
MM DEEP
12
MM DEEP
V
ACUUM REQUIRED
----- 600
MG
H
G
0.8 BAR
WITH COLD TRAP
-----
U
NIT DIMENSIONS
60 X 95 X 100CM 60 X 105 X 140CM 60 X 74 X 83CM 60 X 95 X 100CM
M
EMBRANES SIZE REQUIRED
12.1
X
4.4
CM
11
X
7.4
CM
12.1
X
4.4
CM
12.1
X
4.4
CM
CSL-CAPL Capillary Blotting Unit (28cm x 26cm)
CSL-CAPS Capillary Blotting Unit (15cm x 26cm)
Ordering Information
CSL-D48 48-well Dot Blot Manifold, 3 x 16 array
CSL-D96 96-well Dot Blot Manifold, 8 x 12 array
CSL-S24 24-well Slot Blot Manifold, 2 x 12 array
CSL-S48 48-well Slot Blot Manifold, 3 x 16 array
Ordering Information
Model shown CSL-D48
36
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